PinPoint(TM) Xa-1 T-Vector Sequence This vector can be obtained from Promega Corporation, Madison WI. Call one of the following numbers for order or technical information. Order or Technical 1-800-356-9526 Outside U.S. 608-274-4330 PinPoint(TM) Xa-1 T-Vector sequence reference points: Base pairs 3331 tac promoter 3202-3282 biotin purification tag coding region 1-386 Factor Xa protease recognition site 379-390 multiple cloning region 387-441 PinPoint(TM) Vector Sequencing Primer binding site 325-343 SP6 Promoter Sequencing Primer binding site 451-468 SP6 RNA polymerase promoter (-17 to +3) 449-468 T7 RNA polymerase promoter (-17 to +3) 2796-2815 biotinylated lysine codon 262-264 beta-lactamase (Ampr) coding region 919-1778 The sequence supplied below is that of the circular PinPoint(TM) Xa-1 Vector from which the PinPoint(TM)Xa-1 T-Vector is derived. The PinPoint(TM) Xa-1 Vector has been linearized at base 418 of this sequence (indicated by an asterisk) and a T added to both 3'-ends. The added T is not included in this sequence. The sequence shown corresponds to RNA synthesized by T7 RNA Polymerase and is complementary to RNA synthesized by SP6 RNA Polymerase. The strand shown is the same as the ssDNA strand produced by this vector. 1 ATGAAACTGA AGGTAACAGT CAACGGCACT GCGTATGACG TTGACGTTGA 51 CGTCGACAAG TCACACGAAA ACCCGATGGG CACCATCCTG TTCGGCGGCG 101 GCACCGGCGG CGCGCCGGCA CCGGCAGCAG GTGGCGCAGG CGCCGGTAAG 151 GCCGGAGAGG GCGAGATTCC CGCTCCGCTG GCCGGCACCG TCTCCAAGAT 201 CCTCGTGAAG GAGGGTGACA CGGTCAAGGC TGGTCAGACC GTGCTCGTTC 251 TCGAGGCCAT GAAGATGGAG ACCGAGATCA ACGCTCCCAC CGACGGCAAG 301 GTCGAGAAGG TCCTGGTCAA GGAGCGTGAC GCGGTGCAGG GCGGTCAGGG 351 TCTCATCAAG ATCGGGGATC TCGAGCTCAT CGAAGGTCGC GAAGCTTCAG 401 CTGGGATCCG GTACCGAT*AT CAGATCTCCC GGGGCGGCCG CGATCTGGTT 451 CTATAGTGTC ACCTAAATCG TATGTGTATG ATACATAAGG TTATGTATTA 501 ATTGTAGCCG CGTTCTAACG ACAATATGTC CATATGGTGC ACTCTCAGTA 551 CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGCCCCGACA CCCGCCAACA 601 CCCGCTGACG CGCCCTGACG GGCTTGTCTG CTCCCGGCAT CCGCTTACAG 651 ACAAGCTGTG ACCGTCTCCG GGAGCTGCAT GTGTCAGAGG TTTTCACCGT 701 CATCACCGAA ACGCGCGAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA 751 TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT 801 TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT 851 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA 901 TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT 951 TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC 1001 TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC 1051 ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA 1101 AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG 1151 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC 1201 TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT 1251 TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA 1301 GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG 1351 GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA 1401 TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA 1451 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC 1501 GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC 1551 GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT 1601 TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT 1651 GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC 1701 GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA 1751 TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA 1801 TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA 1851 GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT 1901 TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT 1951 TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC 2001 ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT 2051 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT 2101 CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC 2151 TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG 2201 ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG 2251 GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA 2301 GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA 2351 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC 2401 AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG 2451 GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT 2501 TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC 2551 GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT 2601 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG 2651 AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA 2701 GTGAGCGAGG AAGCGGAAGA GCGCCCAATA CGCAAACCGC CTCTCCCCGC 2751 GCGTTGGCCG ATTCATTAAT GCAGGTTAAC CTGGCTTATC GAAATTAATA 2801 CGACTCACTA TAGGGAGACC GGCCTCGAGC AGCAAGGAGA TGGCGCCCAA 2851 CAGTCCCCCG GCCACGGGGC CTGCCACCAT ACCCACGCCG AAACAAGCGC 2901 TCATGAGCCC GAAGTGGCGA GCCCGATCTT CCCCATCGGT GATGTCGGCG 2951 ATATAGGCGC CAGCAACCGC ACCTGTGGCG CCGGTGATGC CGGCCACGAT 3001 GCGTCCGGCG TAGAGGATCG ATCCGGGCTT ATCGACTGCA CGGTGCACCA 3051 ATGCTTCTGG CGTCAGGCAG CCATCGGAAG CTGTGGTATG GCTGTGCAGG 3101 TCGTAAATCA CTGCATAATT CGTGTCGCTC AAGGCGCACT CCCGTTCTGG 3151 ATAATGTTTT TTGCGCCGAC ATCATAACGG TTCTGGCAAA TATTCTGAAA 3201 TGAGCTGTTG ACAATTAATC ATCGGCTCGT ATAATGTGTG GAATTGTGAG 3251 CGGATAACAA TTTCACACAG GAAACAGAAT TCCCAGCTTG GCTGCAGAAC 3301 CATTCCATTC GTTGATCCGG GAGTAACTCA C Sequence updated 7-Mar-01.