pFN10A (ACT) Flexi(R) Vector This vector can be obtained from Promega Corporation, Madison, WI. Call one of the following numbers for order or technical information. Order or Technical 1-800-356-9526 Outside U.S. 608-274-4330 pFN10A (ACT) Flexi(R) Vector sequence reference points: Base pairs 5867 CMV intermediate/early enhancer/promoter 1-742 Chimeric intron 857-989 T7 RNA Polymerase Promoter (-17 to +3) 1033-1052 VP16 fusion protein 1083-1292 IgA linker 1299-1340 Sgf I region 1332-1339 Barnase coding region 1363-1698 Pme I region 1700-1706 SV40 late polyadenylation signal 1859-2080 SV40 enhancer/early promoter 2179-2597 Neomycin phosphotransferase coding region 2642-3436 Synthetic polyadenylation signal 3500-3548 Beta-lactamase (Ampr) coding region 3809-4669 ColE1-derived plasmid origin of replication 4824-4860 cer region (for E. coli XerCD recombinase) 5531-5816 Note: The IgA linker encodes the polypeptide AIPSTPPTPSPAIA. .. 1 TCAATATTGG CCATTAGCCA TATTATTCAT TGGTTATATA GCATAAATCA 51 ATATTGGCTA TTGGCCATTG CATACGTTGT ATCTATATCA TAATATGTAC 101 ATTTATATTG GCTCATGTCC AATATGACCG CCATGTTGGC ATTGATTATT 151 GACTAGTTAT TAATAGTAAT CAATTACGGG GTCATTAGTT CATAGCCCAT 201 ATATGGAGTT CCGCGTTACA TAACTTACGG TAAATGGCCC GCCTGGCTGA 251 CCGCCCAACG ACCCCCGCCC ATTGACGTCA ATAATGACGT ATGTTCCCAT 301 AGTAACGCCA ATAGGGACTT TCCATTGACG TCAATGGGTG GAGTATTTAC 351 GGTAAACTGC CCACTTGGCA GTACATCAAG TGTATCATAT GCCAAGTCCG 401 CCCCCTATTG ACGTCAATGA CGGTAAATGG CCCGCCTGGC ATTATGCCCA 451 GTACATGACC TTACGGGACT TTCCTACTTG GCAGTACATC TACGTATTAG 501 TCATCGCTAT TACCATGGTG ATGCGGTTTT GGCAGTACAC CAATGGGCGT 551 GGATAGCGGT TTGACTCACG GGGATTTCCA AGTCTCCACC CCATTGACGT 601 CAATGGGAGT TTGTTTTGGC ACCAAAATCA ACGGGACTTT CCAAAATGTC 651 GTAATAACCC CGCCCCGTTG ACGCAAATGG GCGGTAGGCG TGTACGGTGG 701 GAGGTCTATA TAAGCAGAGC TGGTTTAGTG AACCGTCAGA TCACTAGAAG 751 CTTTATTGCG GTAGTTTATC ACAGTTAAAT TGCTAACGCA GTCAGTGCTT 801 CTGACACAAC AGTCTCGAAC TTAAGCTGCA GAAGTTGGTC GTGAGGCACT 851 GGGCAGGTAA GTATCAAGGT TACAAGACAG GTTTAAGGAG ACCAATAGAA 901 ACTGGGCTTG TCGAGACAGA GAAGACTCTT GCGTTTCTGA TAGGCACCTA 951 TTGGTCTTAC TGACATCCAC TTTGCCTTTC TCTCCACAGG TGTCCACTCC 1001 CAGTTCAATT ACAGCTCTTA AGGCTAGAGT ATTAATACGA CTCACTATAG 1051 GGCTAGCCAG CTTGAAGCAA GCCTCCTGAA AGATGAAGCT ACTGTCTTCT 1101 ATCGAACAAG CATGCCCAAA AAAGAAGAGA AAGGTAGATG AATTTCCTGG 1151 GATCTCTACT GCTCCTCCAA CCGATGTCAG CCTGGGCGAC GAACTCCACT 1201 TAGACGGCGA GGACGTGGCG ATGGCGCATG CCGACGCGCT AGACGATTTC 1251 GATCTGGACA TGTTGGGGGA CGGGGATTCC CCGGGTCCGG GATCTCCAGC 1301 GATTCCGTCG ACACCACCTA CTCCCTCTCC AGCGATCGCC ATGGAATAAG 1351 TAAGGAATCC ACATGGCACA GGTTATCAAC ACGTTTGACG GGGTTGCGGA 1401 TTATCTTCAG ACATATCATA AGCTACCTGA TAATTACATT ACAAAATCAG 1451 AAGCACAAGC CCTCGGCTGG GTGGCATCAA AAGGGAACCT TGCAGACGTC 1501 GCTCCGGGGA AAAGCATCGG CGGAGACATC TTCTCAAACA GGGAAGGCAA 1551 ACTCCCGGGC AAAAGCGGAC GAACATGGCG TGAAGCGGAT ATTAACTATA 1601 CATCAGGCTT CAGAAATTCA GACCGGATTC TTTACTCAAG CGACTGGCTG 1651 ATTTACAAAA CAACGGACCA TTATCAGACC TTTACAAAAA TCAGATAATG 1701 TTTAAACGAA TTCGGGCTCG GTACCCGGGG ATCCTCTAGA GTCGACCTGC 1751 AGGCATGCAA GCTGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT 1801 TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCGGCC 1851 GCTTCGAGCA GACATGATAA GATACATTGA TGAGTTTGGA CAAACCACAA 1901 CTAGAATGCA GTGAAAAAAA TGCTTTATTT GTGAAATTTG TGATGCTATT 1951 GCTTTATTTG TAACCATTAT AAGCTGCAAT AAACAAGTTA ACAACAACAA 2001 TTGCATTCAT TTTATGTTTC AGGTTCAGGG GGAGATGTGG GAGGTTTTTT 2051 TAAGCAAGTA AAACCTCTAC AAATGTGGTA AAATCGAATT TTAACAAAAT 2101 ATTAACGCTT ACAATTTCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG 2151 CGGTATTTCA CACCGCATAC GCGGATCTGC GCAGCACCAT GGCCTGAAAT 2201 AACCTCTGAA AGAGGAACTT GGTTAGGTAC CTTCTGAGGC GGAAAGAACC 2251 AGCTGTGGAA TGTGTGTCAG TTAGGGTGTG GAAAGTCCCC AGGCTCCCCA 2301 GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG CAACCAGGTG 2351 TGGAAAGTCC CCAGGCTCCC CAGCAGGCAG AAGTATGCAA AGCATGCATC 2401 TCAATTAGTC AGCAACCATA GTCCCGCCCC TAACTCCGCC CATCCCGCCC 2451 CTAACTCCGC CCAGTTCCGC CCATTCTCCG CCCCATGGCT GACTAATTTT 2501 TTTTATTTAT GCAGAGGCCG AGGCCGCCTC GGCCTCTGAG CTATTCCAGA 2551 AGTAGTGAGG AGGCTTTTTT GGAGGCCTAG GCTTTTGCAA AAAGCTTGAT 2601 TCTTCTGACA CAACAGTCTC GAACTTAAGG CTAGAGCCAC CATGATTGAA 2651 CAAGATGGAT TGCACGCAGG TTCTCCGGCC GCTTGGGTGG AGAGGCTATT 2701 CGGCTATGAC TGGGCACAAC AGACAATCGG CTGCTCTGAT GCCGCCGTGT 2751 TCCGGCTGTC AGCGCAGGGG CGCCCGGTTC TTTTTGTCAA GACCGACCTG 2801 TCCGGTGCCC TGAATGAACT GCAGGACGAG GCAGCGCGGC TATCGTGGCT 2851 GGCCACGACG GGCGTTCCTT GCGCAGCTGT GCTCGACGTT GTCACTGAAG 2901 CGGGAAGGGA CTGGCTGCTA TTGGGCGAAG TGCCGGGGCA GGATCTCCTG 2951 TCATCTCACC TTGCTCCTGC CGAGAAAGTA TCCATCATGG CTGATGCAAT 3001 GCGGCGGCTG CATACGCTTG ATCCGGCTAC CTGCCCATTC GACCACCAAG 3051 CGAAACATCG CATCGAGCGA GCACGTACTC GGATGGAAGC CGGTCTTGTC 3101 GATCAGGATG ATCTGGACGA AGAGCATCAG GGGCTCGCGC CAGCCGAACT 3151 GTTCGCCAGG CTCAAGGCGC GCATGCCCGA CGGCGAGGAT CTCGTCGTGA 3201 CCCATGGCGA TGCCTGCTTG CCGAATATCA TGGTGGAAAA TGGCCGCTTT 3251 TCTGGATTCA TCGACTGTGG CCGGCTGGGT GTGGCGGACC GCTATCAGGA 3301 CATAGCGTTG GCTACCCGTG ATATTGCTGA AGAGCTTGGC GGCGAATGGG 3351 CTGACCGCTT CCTCGTGCTT TACGGTATCG CCGCTCCCGA TTCGCAGCGC 3401 ATCGCCTTCT ATCGCCTTCT TGACGAGTTC TTCTGAGCGG GACTCTGGGG 3451 TTCGAAATGA CCGACCAAGC GACGCCCAAC CTGCCATCAC GATGGCCGCA 3501 ATAAAATATC TTTATTTTCA TTACATCTGT GTGTTGGTTT TTTGTGTGAA 3551 TCGATAGCGA TAAGGATCCT CTTTGCGCTT GCGTTTTCCC TTGTCCAGAT 3601 AGCCCAGTAG CTGACATTCA TCCGGGGTCA GCACCGTTTC TGCGGACTGG 3651 CTTTCTACGT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT 3701 GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA 3751 TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA 3801 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT 3851 GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT 3901 AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG 3951 ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT 4001 CCAATGATGA GCACTTTCAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG 4051 TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA 4101 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC 4151 ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC 4201 TGCGGCCAAC TTACTTCTGA CAACTATCGG AGGACCGAAG GAGCTAACCG 4251 CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA 4301 CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC 4351 TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA 4401 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT 4451 GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA 4501 TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG 4551 GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT 4601 CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC 4651 ACTGATTAAG CATTGGTAAT TCGAAATGAC CGACCAAGCG ACGCCCAACC 4701 GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG 4751 ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC 4801 CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 4851 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG 4901 GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT 4951 CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC 5001 GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG 5051 TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG 5101 CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 5151 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA 5201 GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC 5251 TACGGCTACA CTAGAAGGAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC 5301 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA 5351 CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA 5401 AAAAAAGGAT TTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC 5451 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA 5501 AAAGGATCTT CACCTAGATC CTTTTATAGT CCGGAAATAC AGGAACGCAC 5551 GCTGGATGGC CCTTCGCTGG GATGGTGAAA CCATGAAAAA TGGCAGCTTC 5601 AGTGGATTAA GTGGGGGTAA TGTGGCCTGT ACCCTCTGGT TGCATAGGTA 5651 TTCATACGGT TAAAATTTAT CAGGCGCGAT TGCGGCAGTT TTTCGGGTGG 5701 TTTGTTGCCA TTTTTACCTG TCTGCTGCCG TGATCGCGCT GAACGCGTTT 5751 TAGCGGTGCG TACAATTAAG GGATTATGGT AAATCCACTT ACTGTCTGCC 5801 CTCGTAGCCA TCGAGATAAA CCGCAGTACT CCGGCCACGA TGCGTCCGGC 5851 GTAGAGGATC GAGATCT File updated 21-Jun-06.