pGL4.31[luc2P/GAL4UAS/Hygro] Vector This vector can be purchased from Promega Corporation, Madison, WI. Call one of the following numbers for order or technical information: Ordering or Technical: 1-800-356-9526 Outside U.S.: 608-274-4330 pGL4.31[luc2P/GAL4UAS/Hygro] Vector sequence reference points: GAL4UAS binding region 31-133 Adenovirus major late promoter 145-185 luc2P firefly luciferase reporter 238-2013 SV40 late polyadenylation signal 2053-2274 SV40 enhancer/early promoter 2322-2740 Synthetic hygromycin resistance (Hygror) coding region 2765-3802 Synthetic polyadenylation signal 3826-3874 ColE1-derived plasmid origin of replication 4198-4234 Synthetic Beta-lactamase (Ampr) coding region 4989-5849 Synthetic poly(A) signal/transcriptional pause site 5954-6107 1 GGCCTAACTG GCCGGTACCG AGTTTCTAGA CGGAGTACTG TCCTCCGAGC 51 GGAGTACTGT CCTCCGACTC GAGCGGAGTA CTGTCCTCCG ATCGGAGTAC 101 TGTCCTCCGC GAATTCCGGA GTACTGTCCT CCGAAGACGC TAGCGGGGGG 151 CTATAAAAGG GGGTGGGGGC GTTCGTCCTC ACTCTAGATC TGCGATCTAA 201 GTAAGCTTGG CAATCCGGTA CTGTTGGTAA AGCCACCATG GAAGATGCCA 251 AAAACATTAA GAAGGGCCCA GCGCCATTCT ACCCACTCGA AGACGGGACC 301 GCCGGCGAGC AGCTGCACAA AGCCATGAAG CGCTACGCCC TGGTGCCCGG 351 CACCATCGCC TTTACCGACG CACATATCGA GGTGGACATT ACCTACGCCG 401 AGTACTTCGA GATGAGCGTT CGGCTGGCAG AAGCTATGAA GCGCTATGGG 451 CTGAATACAA ACCATCGGAT CGTGGTGTGC AGCGAGAATA GCTTGCAGTT 501 CTTCATGCCC GTGTTGGGTG CCCTGTTCAT CGGTGTGGCT GTGGCCCCAG 551 CTAACGACAT CTACAACGAG CGCGAGCTGC TGAACAGCAT GGGCATCAGC 601 CAGCCCACCG TCGTATTCGT GAGCAAGAAA GGGCTGCAAA AGATCCTCAA 651 CGTGCAAAAG AAGCTACCGA TCATACAAAA GATCATCATC ATGGATAGCA 701 AGACCGACTA CCAGGGCTTC CAAAGCATGT ACACCTTCGT GACTTCCCAT 751 TTGCCACCCG GCTTCAACGA GTACGACTTC GTGCCCGAGA GCTTCGACCG 801 GGACAAAACC ATCGCCCTGA TCATGAACAG TAGTGGCAGT ACCGGATTGC 851 CCAAGGGCGT AGCCCTACCG CACCGCACCG CTTGTGTCCG ATTCAGTCAT 901 GCCCGCGACC CCATCTTCGG CAACCAGATC ATCCCCGACA CCGCTATCCT 951 CAGCGTGGTG CCATTTCACC ACGGCTTCGG CATGTTCACC ACGCTGGGCT 1001 ACTTGATCTG CGGCTTTCGG GTCGTGCTCA TGTACCGCTT CGAGGAGGAG 1051 CTATTCTTGC GCAGCTTGCA AGACTATAAG ATTCAATCTG CCCTGCTGGT 1101 GCCCACACTA TTTAGCTTCT TCGCTAAGAG CACTCTCATC GACAAGTACG 1151 ACCTAAGCAA CTTGCACGAG ATCGCCAGCG GCGGGGCGCC GCTCAGCAAG 1201 GAGGTAGGTG AGGCCGTGGC CAAACGCTTC CACCTACCAG GCATCCGCCA 1251 GGGCTACGGC CTGACAGAAA CAACCAGCGC CATTCTGATC ACCCCCGAAG 1301 GGGACGACAA GCCTGGCGCA GTAGGCAAGG TGGTGCCCTT CTTCGAGGCT 1351 AAGGTGGTGG ACTTGGACAC CGGTAAGACA CTGGGTGTGA ACCAGCGCGG 1401 CGAGCTGTGC GTCCGTGGCC CCATGATCAT GAGCGGCTAC GTTAACAACC 1451 CCGAGGCTAC AAACGCTCTC ATCGACAAGG ACGGCTGGCT GCACAGCGGC 1501 GACATCGCCT ACTGGGACGA GGACGAGCAC TTCTTCATCG TGGACCGGCT 1551 GAAGAGCCTG ATCAAATACA AGGGCTACCA GGTAGCCCCA GCCGAACTGG 1601 AGAGCATCCT GCTGCAACAC CCCAACATCT TCGACGCCGG GGTCGCCGGC 1651 CTGCCCGACG ACGATGCCGG CGAGCTGCCC GCCGCAGTCG TCGTGCTGGA 1701 ACACGGTAAA ACCATGACCG AGAAGGAGAT CGTGGACTAT GTGGCCAGCC 1751 AGGTTACAAC CGCCAAGAAG CTGCGCGGTG GTGTTGTGTT CGTGGACGAG 1801 GTGCCTAAAG GACTGACCGG CAAGTTGGAC GCCCGCAAGA TCCGCGAGAT 1851 TCTCATTAAG GCCAAGAAGG GCGGCAAGAT CGCCGTGAAT TCTCACGGCT 1901 TCCCTCCCGA GGTGGAGGAG CAGGCCGCCG GCACCCTGCC CATGAGCTGC 1951 GCCCAGGAGA GCGGCATGGA TAGACACCCT GCTGCTTGCG CCAGCGCCAG 2001 GATCAACGTC TAAGGCCGCG ACTCTAGAGT CGGGGCGGCC GGCCGCTTCG 2051 AGCAGACATG ATAAGATACA TTGATGAGTT TGGACAAACC ACAACTAGAA 2101 TGCAGTGAAA AAAATGCTTT ATTTGTGAAA TTTGTGATGC TATTGCTTTA 2151 TTTGTAACCA TTATAAGCTG CAATAAACAA GTTAACAACA ACAATTGCAT 2201 TCATTTTATG TTTCAGGTTC AGGGGGAGGT GTGGGAGGTT TTTTAAAGCA 2251 AGTAAAACCT CTACAAATGT GGTAAAATCG ATAAGGATCC GTTTGCGTAT 2301 TGGGCGCTCT TCCGCTGATC TGCGCAGCAC CATGGCCTGA AATAACCTCT 2351 GAAAGAGGAA CTTGGTTAGC TACCTTCTGA GGCGGAAAGA ACCAGCTGTG 2401 GAATGTGTGT CAGTTAGGGT GTGGAAAGTC CCCAGGCTCC CCAGCAGGCA 2451 GAAGTATGCA AAGCATGCAT CTCAATTAGT CAGCAACCAG GTGTGGAAAG 2501 TCCCCAGGCT CCCCAGCAGG CAGAAGTATG CAAAGCATGC ATCTCAATTA 2551 GTCAGCAACC ATAGTCCCGC CCCTAACTCC GCCCATCCCG CCCCTAACTC 2601 CGCCCAGTTC CGCCCATTCT CCGCCCCATG GCTGACTAAT TTTTTTTATT 2651 TATGCAGAGG CCGAGGCCGC CTCTGCCTCT GAGCTATTCC AGAAGTAGTG 2701 AGGAGGCTTT TTTGGAGGCC TAGGCTTTTG CAAAAAGCTC GATTCTTCTG 2751 ACACTAGCGC CACCATGAAG AAGCCCGAAC TCACCGCTAC CAGCGTTGAA 2801 AAATTTCTCA TCGAGAAGTT CGACAGTGTG AGCGACCTGA TGCAGTTGTC 2851 GGAGGGCGAA GAGAGCCGAG CCTTCAGCTT CGATGTCGGC GGACGCGGCT 2901 ATGTACTGCG GGTGAATAGC TGCGCTGATG GCTTCTACAA AGACCGCTAC 2951 GTGTACCGCC ACTTCGCCAG CGCTGCACTA CCCATCCCCG AAGTGTTGGA 3001 CATCGGCGAG TTCAGCGAGA GCCTGACATA CTGCATCAGT AGACGCGCCC 3051 AAGGCGTTAC TCTCCAAGAC CTCCCCGAAA CAGAGCTGCC TGCTGTGTTA 3101 CAGCCTGTCG CCGAAGCTAT GGATGCTATT GCCGCCGCCG ACCTCAGTCA 3151 AACCAGCGGC TTCGGCCCAT TCGGGCCCCA AGGCATCGGC CAGTACACAA 3201 CCTGGCGGGA TTTCATTTGC GCCATTGCTG ATCCCCATGT CTACCACTGG 3251 CAGACCGTGA TGGACGACAC CGTGTCCGCC AGCGTAGCTC AAGCCCTGGA 3301 CGAACTGATG CTGTGGGCCG AAGACTGTCC CGAGGTGCGC CACCTCGTCC 3351 ATGCCGACTT CGGCAGCAAC AACGTCCTGA CCGACAACGG CCGCATCACC 3401 GCCGTAATCG ACTGGTCCGA AGCTATGTTC GGGGACAGTC AGTACGAGGT 3451 GGCCAACATC TTCTTCTGGC GGCCCTGGCT GGCTTGCATG GAGCAGCAGA 3501 CTCGCTACTT CGAGCGCCGG CATCCCGAGC TGGCCGGCAG CCCTCGTCTG 3551 CGAGCCTACA TGCTGCGCAT CGGCCTGGAT CAGCTCTACC AGAGCCTCGT 3601 GGACGGCAAC TTCGACGATG CTGCCTGGGC TCAAGGCCGC TGCGATGCCA 3651 TCGTCCGCAG CGGGGCCGGC ACCGTCGGTC GCACACAAAT CGCTCGCCGG 3701 AGCGCAGCCG TATGGACCGA CGGCTGCGTC GAGGTGCTGG CCGACAGCGG 3751 CAACCGCCGG CCCAGTACAC GACCGCGCGC TAAGGAGGTA GGTCGAGTTT 3801 AAACTCTAGA ACCGGTCATG GCCGCAATAA AATATCTTTA TTTTCATTAC 3851 ATCTGTGTGT TGGTTTTTTG TGTGTTCGAA CTAGATGCTG TCGACCGATG 3901 CCCTTGAGAG CCTTCAACCC AGTCAGCTCC TTCCGGTGGG CGCGGGGCAT 3951 GACTATCGTC GCCGCACTTA TGACTGTCTT CTTTATCATG CAACTCGTAG 4001 GACAGGTGCC GGCAGCGCTC TTCCGCTTCC TCGCTCACTG ACTCGCTGCG 4051 CTCGGTCGTT CGGCTGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA 4101 TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA 4151 AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT 4201 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA 4251 GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC 4301 CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 4351 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT 4401 CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC 4451 TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA 4501 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG 4551 CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA 4601 GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 4651 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA 4701 GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT 4751 TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT 4801 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG 4851 GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA 4901 AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAAACTTG 4951 GTCTGACAGC GGCCGCAAAT GCTAAACCAC TGCAGTGGTT ACCAGTGCTT 5001 GATCAGTGAG GCACCGATCT CAGCGATCTG CCTATTTCGT TCGTCCATAG 5051 TGGCCTGACT CCCCGTCGTG TAGATCACTA CGATTCGTGA GGGCTTACCA 5101 TCAGGCCCCA GCGCAGCAAT GATGCCGCGA GAGCCGCGTT CACCGGCCCC 5151 CGATTTGTCA GCAATGAACC AGCCAGCAGG GAGGGCCGAG CGAAGAAGTG 5201 GTCCTGCTAC TTTGTCCGCC TCCATCCAGT CTATGAGCTG CTGTCGTGAT 5251 GCTAGAGTAA GAAGTTCGCC AGTGAGTAGT TTCCGAAGAG TTGTGGCCAT 5301 TGCTACTGGC ATCGTGGTAT CACGCTCGTC GTTCGGTATG GCTTCGTTCA 5351 ACTCTGGTTC CCAGCGGTCA AGCCGGGTCA CATGATCACC CATATTATGA 5401 AGAAATGCAG TCAGCTCCTT AGGGCCTCCG ATCGTTGTCA GAAGTAAGTT 5451 GGCCGCGGTG TTGTCGCTCA TGGTAATGGC AGCACTACAC AATTCTCTTA 5501 CCGTCATGCC ATCCGTAAGA TGCTTTTCCG TGACCGGCGA GTACTCAACC 5551 AAGTCGTTTT GTGAGTAGTG TATACGGCGA CCAAGCTGCT CTTGCCCGGC 5601 GTCTATACGG GACAACACCG CGCCACATAG CAGTACTTTG AAAGTGCTCA 5651 TCATCGGGAA TCGTTCTTCG GGGCGGAAAG ACTCAAGGAT CTTGCCGCTA 5701 TTGAGATCCA GTTCGATATA GCCCACTCTT GCACCCAGTT GATCTTCAGC 5751 ATCTTTTACT TTCACCAGCG TTTCGGGGTG TGCAAAAACA GGCAAGCAAA 5801 ATGCCGCAAA GAAGGGAATG AGTGCGACAC GAAAATGTTG GATGCTCATA 5851 CTCGTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ACTAGTACGT 5901 CTCTCAAGGA TAAGTAAGTA ATATTAAGGT ACGGGAGGTA TTGGACAGGC 5951 CGCAATAAAA TATCTTTATT TTCATTACAT CTGTGTGTTG GTTTTTTGTG 6001 TGAATCGATA GTACTAACAT ACGCTCTCCA TCAAAACAAA ACGAAACAAA 6051 ACAAACTAGC AAAATAGGCT GTCCCCAGTG CAAGTGCAGG TGCCAGAACA 6101 TTTCTCT File prepared 21-Jun-06.