phRG-B Vector This vector can be obtained from Promega Corporation, Madison, WI. Call one of the following numbers for order or technical information. Order or Technical 1-800-356-9526 Outside U.S. 608-274-4330 phRG-B Vector Sequence Reference Points: Base pairs 4101 Multiple cloning region 1-58 hRluc reporter gene 88-1023 SV40 late poly(A) region 1055-1276 RVprimer4 binding site 1363-1344 ColE1-derived plasmid replication origin 1601 Beta-lactamase (Ampr) coding region 3223-2363 f1 origin of replication 3356-3810 Synthetic poly(A) signal/transcriptional pause region 3941-4094 RVprimer3 binding site 4043-4062 .. 1 GGTACCGAGC TCTTACGCGT GCTAGCCCGG GCTCGAGATC TGCGATCTAA 51 GTAAGCTTGG CATTCCGGTA CTGTTGGTAA AGCCACCATG GCTTCCAAGG 101 TGTACGACCC CGAGCAACGC AAACGCATGA TCACTGGGCC TCAGTGGTGG 151 GCTCGCTGCA AGCAAATGAA CGTGCTGGAC TCCTTCATCA ACTACTATGA 201 TTCCGAGAAG CACGCCGAGA ACGCCGTGAT TTTTCTGCAT GGTAACGCTG 251 CCTCCAGCTA CCTGTGGAGG CACGTCGTGC CTCACATCGA GCCCGTGGCT 301 AGATGCATCA TCCCTGATCT GATCGGAATG GGTAAGTCCG GCAAGAGCGG 351 GAATGGCTCA TATCGCCTCC TGGATCACTA CAAGTACCTC ACCGCTTGGT 401 TCGAGCTGCT GAACCTTCCA AAGAAAATCA TCTTTGTGGG CCACGACTGG 451 GGGGCTTGTC TGGCCTTTCA CTACTCCTAC GAGCACCAAG ACAAGATCAA 501 GGCCATCGTC CATGCTGAGA GTGTCGTGGA CGTGATCGAG TCCTGGGACG 551 AGTGGCCTGA CATCGAGGAG GATATCGCCC TGATCAAGAG CGAAGAGGGC 601 GAGAAAATGG TGCTTGAGAA TAACTTCTTC GTCGAGACCA TGCTCCCAAG 651 CAAGATCATG CGGAAACTGG AGCCTGAGGA GTTCGCTGCC TACCTGGAGC 701 CATTCAAGGA GAAGGGCGAG GTTAGACGGC CTACCCTCTC CTGGCCTCGC 751 GAGATCCCTC TCGTTAAGGG AGGCAAGCCC GACGTCGTCC AGATTGTCCG 801 CAACTACAAC GCCTACCTTC GGGCCAGCGA CGATCTGCCT AAGATGTTCA 851 TCGAGTCCGA CCCTGGGTTC TTTTCCAACG CTATTGTCGA GGGAGCTAAG 901 AAGTTCCCTA ACACCGAGTT CGTGAAGGTG AAGGGCCTCC ACTTCAGCCA 951 GGAGGACGCT CCAGATGAAA TGGGTAAGTA CATCAAGAGC TTCGTGGAGC 1001 GCGTGCTGAA GAACGAGCAG TAATTCTAGA GTCGGGGCGG CCGGCCGCTT 1051 CGAGCAGACA TGATAAGATA CATTGATGAG TTTGGACAAA CCACAACTAG 1101 AATGCAGTGA AAAAAATGCT TTATTTGTGA AATTTGTGAT GCTATTGCTT 1151 TATTTGTAAC CATTATAAGC TGCAATAAAC AAGTTAACAA CAACAATTGC 1201 ATTCATTTTA TGTTTCAGGT TCAGGGGGAG GTGTGGGAGG TTTTTTAAAG 1251 CAAGTAAAAC CTCTACAAAT GTGGTAAAAT CGATAAGGAT CCGTCGACCG 1301 ATGCCCTTGA GAGCCTTCAA CCCAGTCAGC TCCTTCCGGT GGGCGCGGGG 1351 CATGACTATC GTCGCCGCAC TTATGACTGT CTTCTTTATC ATGCAACTCG 1401 TAGGACAGGT GCCGGCAGCG CTCTTCCGCT TCCTCGCTCA CTGACTCGCT 1451 GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG 1501 TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA 1551 GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 1601 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT 1651 CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT 1701 CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 1751 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA 1801 GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG 1851 GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 1901 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG 1951 CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT 2001 ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT 2051 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG 2101 GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT 2151 GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 2201 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT 2251 AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT 2301 TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC 2351 TTGGTCTGAC AGTTACCAAT GCTTAATCAG TGAGGCACCT ATCTCAGCGA 2401 TCTGTCTATT TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA 2451 ACTACGATAC GGGAGGGCTT ACCATCTGGC CCCAGTGCTG CAATGATACC 2501 GCGAGACCCA CGCTCACCGG CTCCAGATTT ATCAGCAATA AACCAGCCAG 2551 CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG CAACTTTATC CGCCTCCATC 2601 CAGTCTATTA ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA 2651 TAGTTTGCGC AACGTTGTTG CCATTGCTAC AGGCATCGTG GTGTCACGCT 2701 CGTCGTTTGG TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA 2751 GTTACATGAT CCCCCATGTT GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC 2801 TCCGATCGTT GTCAGAAGTA AGTTGGCCGC AGTGTTATCA CTCATGGTTA 2851 TGGCAGCACT GCATAATTCT CTTACTGTCA TGCCATCCGT AAGATGCTTT 2901 TCTGTGACTG GTGAGTACTC AACCAAGTCA TTCTGAGAAT AGTGTATGCG 2951 GCGACCGAGT TGCTCTTGCC CGGCGTCAAT ACGGGATAAT ACCGCGCCAC 3001 ATAGCAGAAC TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA 3051 AAACTCTCAA GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC 3101 TCGTGCACCC AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG 3151 GGTGAGCAAA AACAGGAAGG CAAAATGCCG CAAAAAAGGG AATAAGGGCG 3201 ACACGGAAAT GTTGAATACT CATACTCTTC CTTTTTCAAT ATTATTGAAG 3251 CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT 3301 AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA 3351 CCTGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC 3401 GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG 3451 CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT 3501 CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT 3551 CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC 3601 CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT 3651 AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA 3701 TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA 3751 ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTTAACAA AATATTAACG 3801 CTTACAATTT GCCATTCGCC ATTCAGGCTG CGCAACTGTT GGGAAGGGCG 3851 ATCGGTGCGG GCCTCTTCGC TATTACGCCA GCCCAAGCTA CCATGATAAG 3901 TAAGTAATAT TAAGGTACGG GAGGTACTTG GAGCGGCCGC AATAAAATAT 3951 CTTTATTTTC ATTACATCTG TGTGTTGGTT TTTTGTGTGA ATCGATAGTA 4001 CTAACATACG CTCTCCATCA AAACAAAACG AAACAAAACA AACTAGCAAA 4051 ATAGGCTGTC CCCAGTGCAA GTGCAGGTGC CAGAACATTT CTCTATCGAT 4101 A Sequence file updated 15-May-01.