psiLentGene(TM) Hygromycin Vector This vector can be obtained from Promega Corporation, Madison, WI. Call one of the following numbers for order or technical information. Order or Technical 1-800-356-9526 Outside U.S. 608-274-4330 psiLentGene(TM) Hygromycin Vector Sequence Reference Points: Base pairs 4601 T7 RNA polymerase transcription initiation site 1 Multiple cloning region 10-128 SP6 RNA polymerase promoter (-17 to +3) 139-158 SP6 RNA polymerase transcription initiation site 141 pUC/M13 reverse sequencing primer binding site 176-197 lacZ coding sequence 180-4398 lac operon 200-216 SV40 enhancer and early promoter 410-828 SV40 minimum origin of replication 726-791 Coding region of hygromycin phosphotransferase 863-1888 Synthetic polyadenylation signal 1923-1971 ColE1-derived plasmid replication origin 2161 Beta-lactamase (Ampr) coding region 2923-3783 Phage f1 region 3966-4421 lac operon sequences 4422-4582,166-395 pUC/M13 forward sequencing primer binding site 4535-4558 T7 RNA polymerase promoter (-17 to +3) 4585-3 .. 1 GGGCGAATTG GGCCCGACGT CGCATGCTCC CGGCCGCCAT GGCGGCCGCG 51 GGAATTCGAT ATCACTAGTG AATTCGCGGC CGCCTGCAGG TCGACCATAT 101 GGGAGAGCTC CCAACGCGTT GGATGCATAG CTTGAGTATT CTATAGTGTC 151 ACCTAAATAG CTTGGCGTAA TCATGGTCAT AGCTGTTTCC TGTGTGAAAT 201 TGTTATCCGC TCACAATTCC ACACAACATA CGAGCCGGAA GCATAAAGTG 251 TAAAGCCTGG GGTGCCTAAT GAGTGAGCTA ACTCACATTA ATTGCGTTGC 301 GCTCACTGCC CGCTTTCCAG TCGGGAAACC TGTCGTGCCA GCTGCATTAA 351 TGAATCGGCC AACGCGCGGG GAGAGGCGGT TTGCGTATTG GGCGCTCTTC 401 CGCTGATCTG CGCAGCACCA TGGCCTGAAA TAACCTCTGA AAGAGGAACT 451 TGGTTAGGTA CCTTCTGAGG CGGAAAGAAC CAGCTGTGGA ATGTGTGTCA 501 GTTAGGGTGT GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA AGTATGCAAA 551 GCATGCATCT CAATTAGTCA GCAACCAGGT GTGGAAAGTC CCCAGGCTCC 601 CCAGCAGGCA GAAGTATGCA AAGCATGCAT CTCAATTAGT CAGCAACCAT 651 AGTCCCGCCC CTAACTCCGC CCATCCCGCC CCTAACTCCG CCCAGTTCCG 701 CCCATTCTCC GCCCCATGGC TGACTAATTT TTTTTATTTA TGCAGAGGCC 751 GAGGCCGCCT CGGCCTCTGA GCTATTCCAG AAGTAGTGAG GAGGCTTTTT 801 TGGAGGCCTA GGCTTTTGCA AAAAGCTTGA TTCTTCTGAC GCTAGCGATC 851 GCCCGGGCCA CCATGAAAAA GCCTGAACTC ACCGCGACGT CTGTCGAGAA 901 GTTTCTGATC GAAAAGTTCG ACAGCGTCTC CGACCTGATG CAGCTCTCGG 951 AGGGCGAAGA ATCTCGTGCT TTCAGCTTCG ATGTAGGAGG GCGTGGATAT 1001 GTCCTGCGGG TAAATAGCTG CGCCGATGGT TTCTACAAAG ATCGTTATGT 1051 TTATCGGCAC TTTGCATCGG CCGCGCTCCC GATTCCGGAA GTGCTTGACA 1101 TTGGGGAATT CAGCGAGAGC CTGACCTATT GCATCTCCCG CCGTGCACAG 1151 GGTGTCACGT TGCAAGACCT GCCTGAAACC GAACTGCCCG CTGTTCTGCA 1201 GCCGGTCGCG GAGGCCATGG ATGCGATCGC TGCGGCCGAT CTTAGCCAGA 1251 CGAGCGGGTT CGGCCCATTC GGACCGCAAG GAATCGGTCA ATACACTACA 1301 TGGCGTGATT TCATATGCGC GATTGCTGAT CCCCATGTGT ATCACTGGCA 1351 AACTGTGATG GACGACACCG TCAGTGCGTC CGTCGCGCAG GCTCTCGATG 1401 AGCTGATGCT TTGGGCCGAG GACTGCCCCG AAGTCCGGCA CCTCGTGCAC 1451 GCGGATTTCG GCTCCAACAA TGTCCTGACG GACAATGGCC GCATAACAGC 1501 GGTCATTGAC TGGAGCGAGG CGATGTTCGG GGATTCCCAA TACGAGGTCG 1551 CCAACATCTT CTTCTGGAGG CCGTGGTTGG CTTGTATGGA GCAGCAGACG 1601 CGCTACTTCG AGCGGAGGCA TCCGGAGCTT GCAGGATCGC CGCGGCTCCG 1651 GGCGTATATG CTCCGCATTG GTCTTGACCA ACTCTATCAG AGCTTGGTTG 1701 ACGGCAATTT CGATGATGCA GCTTGGGCGC AGGGTCGATG CGACGCAATC 1751 GTCCGATCCG GAGCCGGGAC TGTCGGGCGT ACACAAATCG CCCGCAGAAG 1801 CGCGGCCGTC TGGACCGATG GCTGTGTAGA AGTACTCGCC GATAGTGGAA 1851 ACCGACGCCC CAGCACTCGT CCGAGGGCAA AGGAATAGCT CGAGTTTAAA 1901 CTCTAGAACC GGTCATGGCC GCAATAAAAT ATCTTTATTT TCATTACATC 1951 TGTGTGTTGG TTTTTTGTGT GTTCGAACTA GAAGCTTGCT TCCTCGCTCA 2001 CTGACTCGCT GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC 2051 TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 2101 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG 2151 CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA 2201 AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA 2251 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC 2301 TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG 2351 CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 2401 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG 2451 CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA 2501 TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT 2551 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA 2601 GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA 2651 AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 2701 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC 2751 AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA 2801 AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC 2851 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT 2901 ATGAGTAAAC TTGGTCTGAC AGTTACCAAT GCTTAATCAG TGAGGCACCT 2951 ATCTCAGCGA TCTGTCTATT TCGTTCATCC ATAGTTGCCT GACTCCCCGT 3001 CGTGTAGATA ACTACGATAC GGGAGGGCTT ACCATCTGGC CCCAGTGCTG 3051 CAATGATACC GCGAGACCCA CGCTCACCGG CTCCAGATTT ATCAGCAATA 3101 AACCAGCCAG CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG CAACTTTATC 3151 CGCCTCCATC CAGTCTATTA ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT 3201 CGCCAGTTAA TAGTTTGCGC AACGTTGTTG CCATTGCTAC AGGCATCGTG 3251 GTGTCACGCT CGTCGTTTGG TATGGCTTCA TTCAGCTCCG GTTCCCAACG 3301 ATCAAGGCGA GTTACATGAT CCCCCATGTT GTGCAAAAAA GCGGTTAGCT 3351 CCTTCGGTCC TCCGATCGTT GTCAGAAGTA AGTTGGCCGC AGTGTTATCA 3401 CTCATGGTTA TGGCAGCACT GCATAATTCT CTTACTGTCA TGCCATCCGT 3451 AAGATGCTTT TCTGTGACTG GTGAGTACTC AACCAAGTCA TTCTGAGAAT 3501 AGTGTATGCG GCGACCGAGT TGCTCTTGCC CGGCGTCAAT ACGGGATAAT 3551 ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG CTCATCATTG GAAAACGTTC 3601 TTCGGGGCGA AAACTCTCAA GGATCTTACC GCTGTTGAGA TCCAGTTCGA 3651 TGTAACCCAC TCGTGCACCC AACTGATCTT CAGCATCTTT TACTTTCACC 3701 AGCGTTTCTG GGTGAGCAAA AACAGGAAGG CAAAATGCCG CAAAAAAGGG 3751 AATAAGGGCG ACACGGAAAT GTTGAATACT CATACTCTTC CTTTTTCAAT 3801 ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT 3851 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG 3901 AAAAGTGCCA CCTGATGCGG TGTGAAATAC CGCACAGATG CGTAAGGAGA 3951 AAATACCGCA TCAGGAAATT GTAAGCGTTA ATATTTTGTT AAAATTCGCG 4001 TTAAATTTTT GTTAAATCAG CTCATTTTTT AACCAATAGG CCGAAATCGG 4051 CAAAATCCCT TATAAATCAA AAGAATAGAC CGAGATAGGG TTGAGTGTTG 4101 TTCCAGTTTG GAACAAGAGT CCACTATTAA AGAACGTGGA CTCCAACGTC 4151 AAAGGGCGAA AAACCGTCTA TCAGGGCGAT GGCCCACTAC GTGAACCATC 4201 ACCCTAATCA AGTTTTTTGG GGTCGAGGTG CCGTAAAGCA CTAAATCGGA 4251 ACCCTAAAGG GAGCCCCCGA TTTAGAGCTT GACGGGGAAA GCCGGCGAAC 4301 GTGGCGAGAA AGGAAGGGAA GAAAGCGAAA GGAGCGGGCG CTAGGGCGCT 4351 GGCAAGTGTA GCGGTCACGC TGCGCGTAAC CACCACACCC GCCGCGCTTA 4401 ATGCGCCGCT ACAGGGCGCG TCCATTCGCC ATTCAGGCTG CGCAACTGTT 4451 GGGAAGGGCG ATCGGTGCGG GCCTCTTCGC TATTACGCCA GCTGGCGAAA 4501 GGGGGATGTG CTGCAAGGCG ATTAAGTTGG GTAACGCCAG GGTTTTCCCA 4551 GTCACGACGT TGTAAAACGA CGGCCAGTGA ATTGTAATAC GACTCACTAT 4601 A Sequence file provided 23-Mar-04.